By Milton, Editor; Gross
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Additional info for Alcohol Intoxication and Withdrawal: Experimental Studies II
The experiments involving labeling of the microsomal fraction with 14c-serine, presented in Sections IV and V, were jointly planned by H. W. and P. V. The laboratory work was mainly carried out or supervised by P. V. II. TOLERANCE INDUCED BY SPACED DOSES VERSUS MAINTAINED ETHANOL INTOXICATION Sprague-Dawley rats obtained from Oy Orion Ab, Helsinki, 4-5 months of age, were maintained on a liquid diet, the composition of which has been described by Wallgren, Kosunen and Ahtee (1973), with the modification that the casein hydrolysate was replaced by milk powder (fatt-free) and the carragenate by lecithin, keeping the protein content unchanged.
Free and bound polysomes were obtained 24 hours later and their radioactive content was determined in the cold TCA insoluble residue as described in the text. Mice were 32 weeks old and drank 10% ethanol or water for 27 weeks. 037 142 Free and membrane bound brain polysomes were isolated as described in the text. Mice were 32 weeks old and drank 10% ethanol or water for 27 weeks. 42 S. TEWARI AND E. P. NOBLE mined on RNA synthesis under in vitro conditions in purified brain cell nuclei. Maximum incorpo~tion of (8-JH)GTP into the cold TCA insoluble residue occurred at the 15 minute time point in both the control and ethanol groups (Fig.
NOBLE of the nucleotide rnixtures of UTP, GTP and CTP (collectively called UGC) incorporation was stirnulated significantly in the RNA systern while a large decrease occurred in the poly A systern. Furtherrnore, while the addition of the DNA in the presence of UGC was stirnulatory to both the RNA and the poly A systern, in the forrner it was additive while in the latter it failed to relieve substantially the rnarked inhibition produced by the addition of the UGC rnixture. Effects of ethanol on (8-3H)ATP incorporation into poly A.
Alcohol Intoxication and Withdrawal: Experimental Studies II by Milton, Editor; Gross
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