By Louis-Marie Houdebine

ISBN-10: 0470848278

ISBN-13: 9780470848272

ISBN-10: 0470862289

ISBN-13: 9780470862285

Animal Transgenesis and Cloning is a concise, balanced creation to this dynamic topic, protecting key matters and present innovations at the moment utilized in animal transgenesis and cloning. when offering the reader with the necessities of the topic, from the molecular foundation of gene constitution and serve as to healing gene cloning in people, social and moral implications of this significant region of study also are thought of. Written in a transparent, available variety, the publication begins with an advent to key molecular biology options and particularly, considers options used in particular for cloning animals and producing transgenic animals. Later chapters research the various theoretical, technical and moral matters raised through cloning and transgenesis in either animals and people. Animal Transgenesis and Cloning: * Is an obtainable, concise advent to this dynamic box * Discusses organic, technical and moral concerns on the topic of cloning and transgenesis * offers an up to date account of the newest study tools and purposes. stories 'This is a suggestion for a e-book translation in a box that's in actual fact of value and intensely topical. the assumption of the booklet, from gene to transgenic animal, to its makes use of and abuses is sound and logical ...... it's possible that i might recommend (using) a booklet just like the single proposed' Dr Janet Smith, collage of Birmingham. 'Chapter one used to be a journey de strength! Very concise and admirably transparent ...... i used to be really inspired with the author's 2-page precis of eukaryotic gene law, that's no suggest achievment!' Dr David de Pomerai, collage of Nottingham. '...... I felt that it is a good written, topical booklet. It covers loads of floor ...... for my part the writer manages to supply a pleasing volume of aspect and a good focussed remedy of every subject. i love the common sense of the booklet, the truth that it pulls jointly all the concerns into one readable and potential textual content' Dr Brendan Curran, Queen Mary, college of London.

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Extra info for Animal Transgenesis and Cloning

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The success of this cloning was initially attributed to the fact that the donor cells were in the G0 phase. It is conceivable, although not easily understandable, that chromatin has a special configuration in G0 which favours genome reprogramming. It turned out not to be as simple as that. The pioneer experiment of Wilmut et al. strongly stimulated the experimenters working in this field. , 1998). Other cloned cows, sheep and goats were obtained in the following months and years. Unexpectedly, the different groups involved in the cloning experiments were all successful, although each of them had its own protocol.

Medecine/Sciences (2000) 16: 1017±1029 Q John Libbey Eurotext. A blastomere isolated from a morula or a blastocyst is inserted between the zona pellucida and cell membrane of a previously enucleated oocyte. 1 CLONING 39 the plasma membranes of the cell and the oocyte. This was achieved by subjecting the material to an alternative electric field. This treatment destabilizes the cell membranes, which spontaneously fuse under these conditions. The nucleus of the cell is thus transferred to the oocyte cytoplasm without any micromanipulation.

Studies are currently being conducted to decipher the mechanism of action of this gene. This may not only provide interesting information on the mechanisms controlling ovulation in mammals; it may also help define new methods to enhance fertility in animals and in humans and to generate new contraceptives. The mutated allele of the BMPR-1B gene may also be transferred to non-hyperprolific sheep and also to goats, cows, pigs and perhaps other domestic species to tentatively enhance their fertility.

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Animal Transgenesis and Cloning by Louis-Marie Houdebine

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