By Zoe A. Wilson

ISBN-10: 0199635641

ISBN-13: 9780199635641

ISBN-10: 0585483795

ISBN-13: 9780585483795

Arabidopsis has lengthy been stated because the 'Botanical Drosophila' with its small genome, low degrees of repetitive DNA, small dimension and quick iteration time it's an awesome molecular genetic instrument for the research of improvement in greater vegetation. Arabidopsis: a realistic Approach presents an advent to lots of the key options required for using Arabidopsis as an experimental approach. It supplies a easy advent to the optimum development stipulations and genetic assets to be had for Arabidopsis, how this fabric may be dealt with, maintained and used. person chapters describe concepts for the id, mapping (using multi-marker traces and recombinant inbreds), and characterisation of alternative mutants through microscopy, molecular cytogenetics and gene expression research. diverse cloning thoughts, utilizing transposons, T-DNA and map place are defined intimately. Sequencing of the Arabidopsis genome could be accomplished in 2000 and bioinformatics are of key value; the instruments which are to be had and the place they are often chanced on on the net are presented.

Show description

Read Online or Download Arabidopsis: A Practical Approach (Practical Approach Series) PDF

Best molecular biology books

Physics of Life: The Physicist's Road to Biology by Clas Blomberg PDF

The aim of the e-book is to offer a survey of the physics that's correct for organic purposes, and likewise to debate what sort of biology wishes physics. The ebook supplies a extensive account of uncomplicated physics, appropriate for the purposes and diverse purposes from houses of proteins to procedures within the telephone to wider issues akin to the mind, the beginning of existence and evolution.

Get Cerebral Signal Transduction: From First to Fourth PDF

A workforce of major specialists comprehensively evaluate and synthesize the newest advances in our figuring out of the position of cerebral signaling in neuronal and cerebral functionality. The authors emphasize the useful position of a number of the signaling pathways in reminiscence, apoptosis and degeneration, melancholy, and drug dependence in either well-being and diseased states.

Peter M. Hollingsworth, Richard M. Bateman, Richard J.'s Molecular systematics and plant evolution PDF

Content material: utilizing organelle markers to clarify the background, ecology and evolution of plant populations / R. A. Ennos, and . .. [et al. ] -- Isolation inside species and the historical past of glacial refugia / C. Ferris, R. A. King and G. M. Hewitt -- using uniparentally inherited basic series repeat markers in plant inhabitants experiences and systematics / J.

Additional info for Arabidopsis: A Practical Approach (Practical Approach Series)

Example text

P. and Acedo, G. (1976). In Cell genetics in higher plants, p. 39. Akad. Kiado, Budapest. 13. Conklin, P. , Williams, E. , and Last, R. L. (1996). Proc. Natl. Acad. Sci. USA, 93, 9970. 14. Relichova, J. (1976). Arabidopsis Inf. , 13, 25. 15. Hirono, Y. and Redei, G. P. (1963). Nature, 197, 1324. 16. Vizir, I. Y. (1988). Arabidopsis Inf. , 26, 55. 17. Mandel, M. , and Yanofsky, M. F. (1992). Nature, 360, 273. 18. , Hanhart, C. , Braaksma, F. , and Feenstra, W. J. (1983). J. , 74, 265. 19. Bowman, J.

Stakes can be made from commercially available garden wire and pea rings. 4. Use the ARACON™ system, a commercial system that provides a plastic collar to surround the plant as it bolts, so that each plant is isolated. An alternative to this system can be made from cutting down clear plastic drinks bottles. D. Harvesting After a period of about three to four weeks in optimal conditions, siliques will start to form. The silique takes about two weeks to mature in which time it turns from green to yellow/brown.

The seed will equilibrate to approximately 5% moisture content within two days to two months depending on the volume of the seed sample, its initial moisture content, and the type of seed container used. Examples of the time taken for different seed lots to reach low seed moisture contents are shown in Table 2. Determination of seed moisture content is described in Protocol 3. When removing seed from archival storage at -20 °C for use, extreme care must be taken to prevent damaging the seeds. Such freeze-thaw cycles should be kept to a minimum.

Download PDF sample

Arabidopsis: A Practical Approach (Practical Approach Series) by Zoe A. Wilson


by Thomas
4.4

Zoe A. Wilson's Arabidopsis: A Practical Approach (Practical Approach PDF
Rated 4.21 of 5 – based on 41 votes